Methods to study the coupling between replicative helicase and leading-strand DNA polymerase at the replication fork
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- 作者：Divya Nandakumar ; Smita S. Patel ; patelss@rutgers.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Replicative helicase ; DNA polymerase ; Pre-steady state kinetics ; Base pair unwinding-synthesis rate constant ; Chemical step size ; Structural biology ; Bacteriophage T7 replication ; Mitochondrial DNA replication
- 刊名：Methods
- 出版年：2016
- 出版时间：1 October 2016
- 年：2016
- 卷：108
- 期：Complete
- 页码：65-78
- 全文大小：2057 K
文摘
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Methods to investigate functional coupling between helicase and leading strand DNAP.
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Presteady state kinetic methods to measure single bp DNA synthesis rate constants.
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Single pot assay to measure the energy consumption of helicase-DNAP complex.
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Methods for positional analysis of helicase and DNAP at the replication fork.
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Contributions of helicase and DNAP to fork junction base pair melting.