Column selectivity in reversed-phase liquid chromatography: IV. Type-B alkyl-silica columns

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• 作者：Gilroy ; Jonathan J. ; Dolan ; John W. ; Snyder ; Lloyd R.
• 关键词：Column selectivity ; Selectivity ; Stationary phases ; LC ; Alkyl-silica columns ; type B ; Hydrophobicity ; Steric selectivity ; Hydrogen bond acidity ; Hydrogen bond basicity ; Cation-exchange behavior
• 刊名：Journal of Chromatography A
• 出版年：2003
• 出版时间：June 6, 2003
• 年：2003
• 卷：1000
• 期：1-2
• 页码：757-778
• 全文大小：351 K

文摘

Columns for reversed-phase HPLC (RP-LC) can be characterized by five, retention-related parameters: H (hydrophobicity), S (steric selectivity), A (hydrogen-bond acidity), B (hydrogen-bond basicity), and C (cation-exchange behavior). In the present study, values of the latter parameters have been measured for 92 type-B (low metals content) alkyl-silica columns and compared to column properties such as ligand length, ligand concentration, pore diameter, and the presence or absence of end-capping. With the exception of five columns of unusual design, retention factors, k, for 16 representative test compounds were correlated with values of H, S, etc., within an average ±1.2 % (1 standard deviation, SD), suggesting that all significant solute–column interactions are recognized by these five column parameters. A single-valued function F_s is proposed to measure differences in selectivity for any two RP-LC columns whose values of H, S, etc., are known. This allows the easy selection of columns whose selectivity is desired to be either similar to or different from a starting column, for application in either routine analysis or method development.