Optimizing selectivity during reversed-phase high performance liquid chromatography method development: Prioritizing experimental conditions
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- 作者：Lloyd R. Snyder ; John W. Dolan
- 关键词：Optimization ; Selectivity ; HPLC ; Experimental conditions ; Experimental design ; Method development
- 刊名：Journal of Chromatography A
- 出版年：2013
- 出版时间：9 August, 2013
- 年：2013
- 卷：1302
- 期：Complete
- 页码：45-54
- 全文大小：1331 K
文摘
Several different conditions can be varied to improve selectivity for reversed-phase chromatography (RPC). A reexamination of literature data suggests that changes in selectivity due to a change of column or mobile phase pH are largely replicated by changes in temperature or mobile phase composition (concentrations of acetonitrile and/or methanol). This suggests a reconsideration of the role of mobile phase pH and the column during method development.