Identification of AgNORs and cytopathological changes in oral lichen planus lesions

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• 作者：Stefâ ; nia Jeronimo Ferreira^a ; ^{s.j.f_1985@hotmail.com} ; Maria  ; ngela Naval Machado^b ; ^{man.machado@ufpr.br} ; Antô ; nio Adilson Soares de Lima^b ; ^{antollima@hotmail.com} ; Aline Cristina Batista Rodrigues Johann^a ; ^{aline.johann@pucpr.br} ; Ana Maria Trindade Gré ; gio^a ; ^{ana.gregio@pucpr.br} ; Luciana Reis Azevedo-Alanis^a ; ^{l.azevedo@pucpr.br}
• 关键词：Oral mucosa ; Oral lichen planus ; Cytology ; Precancerous lesions
• 刊名：Acta Histochemica
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：119
• 期：1
• 页码：32-38
• 全文大小：1519 K
• 卷排序：119

文摘

To evaluate cytopathological changes in epithelial cells of the oral mucosa of patients with oral lichen planus (OLP) compared with patients without OLP.Subjects and methodsSwabs were collected from the oral mucosa of 20 patients with OLP (case group) and 20 patients without OLP (control group) using liquid-based cytology. After Papanicolaou staining, the smears were characterized based on Papanicolaou classification and degree of maturation. Nuclear area (NA) measurements, cytoplasmic area (CA) measurements, and the NA/CA ratio were determined from 50 epithelial cells per slide. For quantification of argyrophilic nucleolar organizer regions (AgNORs), the smears were stained with silver nitrate, and the number of AgNORs was counted in 100 cells.ResultsIn both groups, there was a predominance of Papanicolaou Class I nucleated cells in the superficial layer. The average values of NA (p > 0.05) and CA (p = 0.000) were greater in the case group (NA = 521.6, CA = 22,750.3) compared with the control group (NA = 518.9, CA = 18,348.0). The NA/CA ratio was 0.025 for the case group and 0.031 for the control group (p = 0.004). There was no significant difference between the mean AgNORs values of both groups (p > 0.05).ConclusionThe oral mucosa of patients with OLP exhibited significant cytomorphometric changes. However, there was no evidence of malignancy.