Exploration of the therapeutic potential effect of Sepia officinalis in animal model of sepsis induced by cecal ligation and puncture
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- 作者：M.Sc. Asmaa Abd El-Aleem Abd El-Latif ; chem.asmaa19@yahoo.com ; Dr. Amany Ahmed Sayed amanyasayed@sci.cu.edu.eg (Lecturer of Physiology) ; Dr. Amel Mahmoud Soliman soliman.amel5@gmail.com (Professor of Physiology) ; Dr. Sohair Ramadan Fahmy sohairfahmy@gmail.com (Professor of Physiology)
- 关键词：Sepsis ; Sepia officinalis ; Oxidative stress ; Histopathology ; Liver ; Kidney
- 刊名：Injury
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：47
- 期：12
- 页码：2709-2717
- 全文大小：2782 K
- 卷排序：47
文摘
The present investigation explored the therapeutic potential effect of Sepia officinalis body tissue (SOBT) and Sepia officinalis polysaccharide (SOP) extracts, in animal model of sepsis [induced by cecal ligation and puncture (CLP)].Materials and methodsExperimental animals were divided into 4 groups, Group 1: Sham control rats. Group 2: Septic rats. Group 3: Septic rats treated with methanolic extract of Sepia officinalis body tissue (SOBT) (500 mg/kg body weight) for 2 days. Group 4: Septic rats treated with Sepia officinalis polysaccharide (SOP) extract (200 mg/kg body weight) for 2 days.ResultsThe antioxidant activity of SOBT and SOP was proven by DPPH test. CLP-induced liver and kidney toxicities showed by an increase in the ALAT, ASAT, γGT, ALP, creatinine, BUN and uric acid concentrations in serum. Moreover, CLP-induced oxidative stress in liver and kidney evidenced by the increase of MDA levels, decrease in GSH concentrations and decrease in the enzymatic antioxidants (SOD, CAT, GST). In addition, CLP caused decrease in CYP1A2 content in liver.ConclusionsOur findings demonstrate the therapeutic efficacy of SOBT and SOP in liver and kidney disorders. Therefore this study suggests that SOBT and SOP could be a potential therapeutic agents for sepsis treatment.