Identification, cloning and sequence of the Aspergillus niger areA wide domain regulatory gene controlling nitrogen utilisation

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• 作者：MacCabe ; Andrew P. ; Vanhanen ; Sipo ; Sollewijn Gelpke ; Maarten D. ; van de Vondervoort ; Peter J.I. ; et. al.
• 关键词：areA ; Nitrogen metabolite repression ; GATA factor ; Gene regulation ; (Aspergillus niger)
• 刊名：Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression
• 出版年：1998
• 出版时间：March 9, 1998
• 年：1998
• 卷：1396
• 期：2
• 页码：163-168
• 全文大小：522 K

文摘

The gene encoding the positive-acting regulator of nitrogen metabolite repression (AREA) has been cloned and characterised from the industrially important filamentous fungus Aspergillus niger. The deduced amino acid sequence has an overall level of identity with its homologues from other fungal species which varies between 32 and 72 % . This gene (areA_nig) complements the A. nidulans areA^r-18 loss-of-function mutation. Sequences upstream of the structural gene contain several putative GATA-type zinc finger protein-binding motifs. Northern analysis indicates the synthesis of multiple transcripts, the major species being approximately 2.95 kb and 3.1 kb. Maximal expression of areA_nig is observed under conditions of nitrogen starvation and is mainly due to an increase in the level of the shorter transcript.