Fluoro-D-glucose-micro positron emission tomography as a diagnostic tool to confirm brain death in a murine donor lung injury model

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• 作者：Shana Wauters ; MSc^a ; Michel Koole ; PhD^b ; Peter Vermaelen ; BSc^b ; Jana Somers ; MSc^a ; Koen Van Laere ; MD ; PhD^b ; ^c ; Johannes Van Loon ; MD ; PhD^d ; Geert M. Verleden ; MD ; PhD^e ; Dirk Van Raemdonck ; MD ; PhD^a ; ^f ; ^{dirk.vanraemdonck@uzleuven.be}
• 关键词：Brain death ; Positron emission tomography ; Mouse ; Donor lung injury
• 刊名：Journal of Surgical Research
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：180
• 期：2
• 页码：343-348
• 全文大小：675 K

文摘

Purpose

Because brain death (BD)-related donor lung injury is still poorly understood, a reliable mouse model can help in understanding the immunologic mechanisms behind this lung injury. The purpose of our study was to validate BD in mice using small-animal positron emission tomography.

Procedures

BD was induced in male Balb/c mice (27.1 ¡À 0.9 g) with an intracranial balloon catheter inflated rapidly (<1 min) [BD]^R or gradually (36 ¡À 5 min) [BD]^G, and compared with sham-operated [SH] and control animals [C] (n = 6/group). Ten minutes after balloon insertion 10.4 ¡À 1.0 MBq 2-deoxy-2-[¹⁸F]-fluoro-D-glucose (¹⁸FDG) was administered intravenously and static images were performed and quantified.

Results

Coronal, sagittal, and transaxial sections of cerebral ¹⁸FDG activity revealed significant differences when comparing [BD]^R and [BD]^G with [C] and [SH] animals. No significant ¹⁸FDG uptake was visually detectable in [BD]^R and [BD]^G.

The percentage injected dose showed significant differences between BD groups and [C] and [SH] (P < 0.0001). No significant difference was seen between [C] versus [SH] nor between [BD]^R versus [BD]^G (P > 0.05).

Conclusions

¹⁸FDG micro positron emission tomography imaging is a valuable tool to demonstrate brain functionality and can therefore be used as a surrogate test to confirm BD in mice.