Direct electrochemistry of cytochrome c immobilized on a novel macroporous gold film coated with a self-assembled 11-mercaptoundecanoic acid monolayer

详细信息    查看全文

• 作者：Ying Li ; Jing Li ; Xing-Hua Xia ; Song-Qin Liu
• 关键词：Direct electrochemistry ; Cytochrome c ; Macroporous gold ; Hydrogen bubble template ; Galvanic replacement reaction ; Self-assembled monolayer
• 刊名：Talanta
• 出版年：2010
• 出版时间：15 September 2010
• 年：2010
• 卷：82
• 期：4
• 页码：1164-1169
• 全文大小：503 K

文摘

We have successfully constructed a novel gold film with open interconnected macroporous walls of nanoparticles by combining the hydrogen bubble dynamic template synthesis with galvanic replacement reaction. After modified by a self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid (MUA), the three-dimensionally (3D) interconnected macroporous Au film has been used as a biocompatible substrate for the immobilization of cytochrome c. The morphology, structure and electrochemical features of the modified and unmodified macroporous Au films were characterized by field-emission scanning electron microscopy (FESEM), energy-dispersive X-ray (EDX), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results reveal that the resultant films had a large electroactive surface area for high protein loading, enhanced electron transfer of cytochrome c, retained electrochemical activity, good stability and repeatability. And the excellent electrochemical behaviors could be attributed to the hierarchical structure of the macroporous Au film constructed by nanoparticles.