Fluorescence turn-on probes for intracellular RNA distribution and their imaging in confocal and two-photon fluorescence microscopy
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- 作者：Guofen Song ; Fang Miao ; Yuming Sun ; Xiaoqiang Yu ; Jing Zhi Sun ; Wai-Yeung Wong
- 关键词：RNA ; Biosensors ; Two-photon fluorescence ; Bioimaging ; Counterstain compatibility
- 刊名：Sensors and Actuators B: Chemical
- 出版年：2012
- 出版时间：October, 2012
- 年：2012
- 卷：173
- 期：Complete
- 页码：329-337
- 全文大小：1872 K
文摘
We have synthesized four indole derivatives (INS1, IND1, INS2 and IND2) successfully, and experimental results of biochemical RNA fluorescence assays and direct intracellular imaging demonstrated that they are potential highly RNA-selective fluorescent turn-on probes possessing good counterstain compatibility with the classic DNA-staining dye DAPI. Moreover, these sensors exhibit large two-photon excited fluorescence action absorption cross-section in the presence of RNA and are also capable of imaging intracellular RNA in two-photon fluorescence microscopy.