Investigation of the inclusion behavior of HP-¦Â-cyclodextrin with polydatin in solution and its analytical application

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• 作者：Songsong An ; Jiang He ; ^{hejiang@lzu.edu.cn} ; Lijuan Sun ; Dong Ren ; Yihe Ban
• 关键词：Polydatin ; HP&ndash ; ¦Â-cyclodextrin ; Spectrofluorimetry ; Molecular modeling ; Sensitive determination
• 刊名：Journal of Molecular Structure
• 出版年：2013
• 出版时间：10 April, 2013
• 年：2013
• 卷：1037
• 期：Complete
• 页码：9-14
• 全文大小：561 K

文摘

The inclusion complex of the slight water solubility, polydatin (PD), with hydroxypropyl-¦Â-cyclodextrin (HP-¦Â-CD) was investigated for the first time in solution. The effect of HP-¦Â-CD on the spectral feature of PD was measured using fluorescence technique by varying the concentrations of HP-¦Â-CD. Meanwhile, the molecular mechanism of the formation of inclusion complex of PD with HP-¦Â-CD was studied and discussed by molecular modeling. The experimental result clearly indicated that the benzene ring of PD was encapsulated within the HP-¦Â-CD cavity to form a 1:1 stoichiometry host-guest compound. The apparent formation constants (K) was obtained by the typical double reciprocal plots and phase solubility studies. Due to the remarkable enhancement of the fluorescence intensity of PD, a spectrofluorimetric method was developed for the determination of PD in bulk aqueous solution in the presence of HP-¦Â-CD. The linear range was 2.00 ¡Á 10^?7-2.00 ¡Á 10^?5 mol/L (r = 0.998) with the detection limit 9.50 ¡Á 10^?9 mol/L. The present study provided useful information for a more rational determination of PD in serum with recoveries of 97-104 % .