IL-6 UPREGULATES ITS OWN RECEPTOR ON SOME HUMAN MYELOMA CELL LINES

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• 作者：Thabard ; Wilfrid ; Collette ; Madeleine ; Mellerin ; Marie-Paule ; Puthier ; Denis ; Barill&eacute ; Sophie ; et. al.
• 刊名：Cytokine
• 出版年：2001
• 出版时间：June, 2001
• 年：2001
• 卷：14
• 期：6
• 页码：352-356
• 全文大小：281 K

文摘

Interleukin 6 (IL-6) is the major survival factor of myeloma cells. In this study, we demonstrate that IL-6, oncostatin M (OSM) and leukemia inhibitory factor (LIF) upregulate membrane IL-6 receptor α (IL-6Rα) on OPM-2 myeloma cell line at transcriptional level. In OPM-2 cells, IL-6, OSM and LIF induce both signal transducers and activators of transcription (STAT), mitogen activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI 3-K) activation. We show that the cytokine-induced upregulation of IL-6Rα can be abolished by a janus kinase (JAK)-2 specific inhibitor, i.e. AG490, suggesting an involvement of the JAK/STAT pathway in this process. Finally, IL-6Rα upregulation was also inhibited by wortmannin, an inhibitor of the PI 3-kinase pathway. In conclusion, IL-6 can upregulate its own receptor on OPM-2 cells probably through the JAK/STAT and PI 3-kinase pathways.