Two-dimensional liquid chromatography coupled to tandem mass spectrometry for vitamin D metabolite profiling including the C3-epimer-25-monohydroxyvitamin D3
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- 作者：A. Mena-Bravoa ; b ; c ; F. Priego-Capotea ; b ; c ; q72prcaf@uco.es" class="auth_mail" title="E-mail the corresponding author ; M.D. Luque de Castroa ; b ; c ;
- 关键词：1 ; 25(OH)2D2 ; 1 ; 25-dihydroxyvitamin D2 ; 1 ; 25(OH)2D3 ; 1 ; 25-dihydroxyvitamin D3 ; 24 ; 25(OH)2D3 ; 24 ; 25-dihydroxyvitamin D3 ; 25(OH)D2 ; 25-hydroxyvitamin D2 ; 25(OH)D ; 325-hydroxyvitamin D3 ; 3-epi-25(OH)D3 ; 3-epi-25-hydroxyvitamin D3 ; ACE ; automatic cartridge exchange ; CPB ; competitive protein binding assay ; DEQAS ; vitamin D external quality assurance scheme ; ELISA ; enzyme linked immune sorbent assay ; ESI+ ; electrospray ionization in positive mode ; HPD ; shigh-pressure syringe dispensers ; LC&ndash ; MS/MS
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：17 June 2016
- 年：2016
- 卷：1451
- 期：Complete
- 页码：50-57
- 全文大小：1397 K
文摘
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Automated SPE-2DLC–MS/MS method for analysis of vitamin D and metabolites in serum.
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The method provides quantitative information of the C3 epimer of 25(OH)D3.
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SIL-ISs allowed reducing ionization suppression effects and variability sources.
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The method was validated by a standard reference material and according to DEQAS.