Preferential proliferation of attenuated salmonella typhimurium within neuroblastoma

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• 作者：Lel ; J. Soto ; Brent S. Sorenson ; Brent W. Nelson ; Scott J. Solis ; Arnold S. Leonard ; Daniel A. Saltzman
• 关键词：Salmonella typhimurium ; cancer ; interleukin-2 ; neuroblastoma ; osteosarcoma ; hepatocytes
• 刊名：Journal of Pediatric Surgery
• 出版年：2004
• 出版时间：June 2004
• 年：2004
• 卷：39
• 期：6
• 页码：937-940
• 全文大小：98 K

文摘

Attenuated Salmonella typhimurium, a facultative intracellular parasite that colonizes the liver, has been shown to accumulate within extrahepatic malignancies. The authors sought to define a mechanism for attenuated Salmonella accumulation within cancer cells compared with hepatocytes.

Methods

Invasion and intracellular proliferation of attenuated Salmonella was assessed through an in vitro assay performed on neuroblasoma, osteosarcoma, hepatoma, and colon adenocarcinoma cell lines and compared with freshly isolated mouse hepatocytes.

Results

The efficiency of attenuated S typhimurium invasion into hepatocytes was greater than any malignant cell line (3.8 v 0.46; P < .04). However, the intracellular proliferation of the bacteria was most abundant within neuroblastoma, exceeding the proliferation within hepatocytes (14.3 v 6.2; P < .01).

Conclusions

Attenuated S typhimurium may prove to be an effective in vivo immunotherapy for the local delivery of therapeutic proteins to neuroblastoma.