Protein tyrosine nitration in the cell cycle

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• 作者：Min Jia  ; ;   ; ^{min.jia@ki.se} ; Claudia Mateoiu ; Serhiy Souchelnytskyi
• 关键词：Tyrosine nitration ; Cell cycle ; Two-dimensional gel electrophoresis ; Proteomics
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2011
• 出版时间：23 September 2011
• 年：2011
• 卷：413
• 期：2
• 页码：270-276
• 全文大小：662 K

文摘

Nitration of tyrosine residues in proteins is associated with cell response to oxidative/nitrosative stress. Tyrosine nitration is relatively low abundant post-translational modification that may affect protein functions. Little is known about the extent of protein tyrosine nitration in cells during progression through the cell cycle. Here we report identification of proteins enriched for tyrosine nitration in cells synchronized in G0/G1, S or G2/M phases of the cell cycle. We identified 27 proteins in cells synchronized in G0/G1 phase, 37 proteins in S phase synchronized cells, and 12 proteins related to G2/M phase. Nineteen of the identified proteins were previously described as regulators of cell proliferation. Thus, our data indicate which tyrosine nitrated proteins may affect regulation of the cell cycle.