- 作者：Bakul I. Dalal ; Soudeh Mansoor ; Mita Manna ; Steven Pi ; Giovanna Di Sauro ; Donna E. Hogge
- 关键词：Acute myeloid leukemia ; Flow cytometry ; FLT3 ; Normal cytogenetics ; NPM1
- 刊名：Clinical Lymphoma Myeloma and Leukemia
- 出版年：2012
- 出版时间：August, 2012
- 年：2012
- 卷：12
- 期：4
- 页码：274-279
- 全文大小：306 K
Methods
We retrospectively correlated NPM1 and FLT3 mutation status with flow cytometric profile of leukemic blasts in 83 adult patients with cytogenetically normal acute myeloid leukemia (CN-AML).Results
Mutation of the NPM1 gene (NPM1.mt) was found in 39 (47 % ) of 83 patients, and internal tandem duplication (ITD) of the FLT3 gene (FLT3-ITD) was seen in 38 (46 % ) of 83 patients. Patients with CN-AML and with NPM1.mt were less likely to express CD34 (33 % vs. 93 % ; 2P = .0001), CD2 (0 % vs. 14 % ; 2P = .0187), and CD14 (6 % vs. 22 % , 2P = .0476), and were more likely to express CD4 (65.5 % vs. 37 % ; 2P = .0367) and CD19 (49 % vs. 27 % ; 2P = .0506). The patients with CN-AML and with FLT3-ITD were more likely to express CD56 (47 % vs. 23 % ; 2P = .0393). Moreover, patients with favorable prognostic combination of NPM1.mt and wild-type (wt) FLT3 (n = 18) were less likely to express CD34 (33 % vs. 74 % all others; 2P = .0021) and CD56 (6 % vs. 37 % all others; 2P = .0072). The group with an unfavorable prognostic combination of NPM1-wt and FLT3-ITD (n = 17) were more likely to express CD34 (88 % vs. 45 % all others; 2P = .0011) and TdT (40 % vs. 2 % all others; 2P = .0054).
Conclusions
In patients with CN-AML, characteristic flow cytometric profile is associated with NPM1 and FLT3 mutation status.