Establishment of a continuous cell line from southern bluefin tuna (Thunnus maccoyii)

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• 作者：Peter A. Bain^a ; ¹ ; Rhonda G. Hutchinson^b ; Alexandra B. Marks^a ; ^b ; Mark St.J. Crane^c ; Kathryn A. Schuller^a ; ^{kathy.schuller@flinders.edu.au}
• 关键词：Cell line ; Karyotype ; Southern bluefin tuna ; Thunnus maccoyii
• 刊名：Aquaculture
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：376-379
• 期：Complete
• 页码：59-63
• 全文大小：616 K

文摘

A continuous cell line, designated SBT-E1, was established from tissues of a southern bluefin tuna (Thunnus maccoyii) fingerling. Maximum cell proliferation rates occurred at 25 ¡ãC in Liebovitz's L-15 medium containing 10-20 % FBS. Survival of SBT-E1 after short-term cryostorage was 70.8 ¡À 12.8 % . The species of origin was confirmed by sequencing a region of the mitochondrial gene for cytochrome c oxidase, subunit I (cox1). The SBT-E1 karyotype, with a modal chromosome number of 2n = 46, differs from the constitutional karyotype of the parent species (2n = 48), with the apparent loss of either whole or partial chromosomes and the presence of small marker chromosomes. The SBT-E1 cell line represents the first reported cell line from any species of tuna.