Anti-inflammatory intestinal activity of Abarema cochliacarpos (Gomes) Barneby & Grimes in TNBS colitis model

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• 作者：Maria Silene da Silva ; Susana Sá ; nchez-Fidalgo ; Elena Talero ; Ana Cá ; rdeno ; Marcelo Aparecido da Silva ; Wagner Villegas ; Alba Regina Monteiro Souza Brito ; Catalina Alarcó ; n de La Lastra
• 关键词：Abarema cochliacarpos (Mimosaceae) ; Colitis ; Neutrophils ; Cytokines ; Inducible nitric oxide synthase ; Cyclooxygenase ; c-Jun N-terminal kinase
• 刊名：Journal of Ethnopharmacology
• 出版年：2010
• 出版时间：24 March 2010
• 年：2010
• 卷：128
• 期：2
• 页码：467-475
• 全文大小：885 K

文摘

Aim of the study

To assess the anti-inflammatory effect of butanolic fraction of methanolic extract from bark of Abarema cochliacarpos in acute ulcerative colitis model induced by intracolonic administration of trinitrobenzene sulfonic acid (TNBS) in Wistar rats.

Materials and methods

Abarema cochliacarpos (100 and 150 mg/kg/day) was administered by gavage 48, 24 and 1 h prior to the induction of colitis with 10 mg/kg of TNBS and, 24 h later.

Results

Phytochemical studies by mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) revealed that catechins were a major component into condensate class of tannins. Treatment with Abarema cochliacarpos decreased significantly macroscopic damage as compared with TNBS (p < 0.05). Histological analysis showed that both doses of the extract improved the microscopic structure and preserved some areas of the colonic mucosa structure. In addition, myeloperoxidase activity (MPO), as a marker of neutrophil infiltration, was decreased in a dose-dependent way (p < 0.01 and p < 0.001 respectively), TNF-α level was also diminished with the highest dose of the extract (p < 0.001) and, IL-10 level obtained no significant results. In order to elucidate some of the mechanisms, expression of inducible inflammatory enzymes, such as cyclooxygenase (COX)-2 and nitric oxide synthase (iNOS), were studied showing a significant reduction. Finally, the involvement of c-Jun N-terminal kinase (JNK) signalling demonstrated a reduction in the JNK activation with the highest dose (p < 0.05 vs TNBS).

Conclusions

We have shown for the first time that the extracts obtained from Abarema cochliacarpos bark possess active substances, which exert marked protective effects in acute experimental colitis, confirming and justifying, at least in part, the popular use of this plant to treat gastrointestinal diseases.