Molecular cloning, characterization and expression of Lc-Sox11a in large yellow croaker Larimichthys crocea
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- 作者：Yonghua Jianga ; b ; Kunhuang Hanb ; c ; Shihai Chenb ; Wanshu Honga ; Yilei Wangb ; ylwang@jmu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Ziping Zhangd
- 关键词：SRY ; sex-determining region of the Y chromosome ; Lc-Sox11a ; Sox11a of Larimichthys crocea ; RACE ; rapid amplification of cDNA ends ; UTR ; untranslated region ; qRT-PCR ; quantitative real-time PCR ; WISH ; whole mount in situ hybridization ; Sox ; Sry-box ; HMG ; high mobility group ; dph ; days post hatching ; PFA ; Paraformaldehyde-PBS fixation buffer ; DIG ; digoxigenin ; ORF ; open reading frame ; Lc-Sox11a ; encoded the protein by Lc-Sox11a ; ATG ; methionine start codon ; Ser ; serine ; Thr ; threonine ; Tyr ; tyrosi
- 刊名：Gene
- 出版年：2015
- 出版时间：15 December 2015
- 年：2015
- 卷：574
- 期：2
- 页码：287-301
- 全文大小：6346 K
文摘
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A full length cDNA of Lc-sox11a was cloned and analyzed from Larimichthys crocea.
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The expression of Lc-Sox11a had tissue and gender specificity.
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The significant differences of Lc-Sox11a expressed in gonad and brain with growth.
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Lc-Sox11a widely distributed in cytoplasm of germ cells.
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WISH showed Lc-Sox11a mainly expressed in brain and eye areas in embryogenesis.