Cloning and characterization of mouse mSox13 cDN
详细信息 查看全文
- 作者：Kido ; Susumu ; Hiraoka ; Yoshiki ; Ogawa ; Motoyuki ; Sakai ; Yukinao ; Yoshimura ; Yasunori ; Aiso ; Sadakazu
- 关键词：DNA binding ; Embryo ; HMG box ; Leucine zipper ; SRY ; aa ; amino acid(s) ; b ; base(s) ; cDNA ; complementary DNA ; EMSA ; electrophoretic mobility shift assay ; GST ; glutathione-S-transferase ; kb ; kilobase(s) or 1000b ; nt ; nucleotide(s) ; ORF ; open reading frame ; PC
- 刊名：Gene
- 出版年：1998
- 出版时间：February 22, 1998
- 年：1998
- 卷：208
- 期：2
- 页码：201-206
- 全文大小：460 K
文摘
A novel SRY-related cDNA, mSox13, was isolated from a λ phage library derived from mouse embryo. The cDNA encodes a protein of 595 amino acids containing the SRY-type high mobility group (HMG) box and a putative leucine zipper motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named Q box, are well conserved among known type-D SOX proteins. The expression of mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay indicates that the recombinant mSox13 protein is capable of binding to the AACAAT sequence.