Surface characterization of 7S and 11S globulin powders from soy protein examined by X-ray photoelectron spectroscopy and scanning electron microscopy

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• 作者：Xiaoyan Zhao^a ; ^{zhaoxy_2201@163.com"" rel=""nofollow} ; Jun Chen^a ; Qingjun Zhu^a ; Fangling Du^a ; ^{Fanglingdu@yahoo.com.cn"" rel=""nofollow} ; Qiang Ao^b ; Jie Liu^c
• 关键词：Reverse micelle ; XPS ; SEM ; 7S and 11S globulin powder ; Surface composition
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2011
• 出版时间：1 September 2011
• 年：2011
• 卷：86
• 期：2
• 页码：260-266
• 全文大小：523 K

文摘

In this study the surface composition of 7S and 11S globulin powders from soybean proteins by aqueous buffer and reverse micelle extractions had been examined using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Analysis by XPS revealed that the O and N atomic percentage of 7S and 11S globulin surfaces from bis(2-ethylhexyl) sodium sulfosuccinate (AOT) reverse micelle was higher than from aqueous buffer, but the C atomic percentage was lower. The O/C ratio of the 7S globulin powder from aqueous buffer and reverse micelle was similar while significant differences were obtained in the O/C ratio of the 11S globulin powder, N/C atom ratios of the 7S and 11S globulin powders and high-resolution XPS C 1s, N 1s, O 1s spectra. Powder microstructure after reverse micelle treatment showed the presence of small pores, indicating the effect of reverse micelle on the 7S and 11S globulin structure. The obtained results indicated that the reverse micelle could affect the C, O and N components on the surface of soybean proteins.