Analgesic effect of total flavonoids from Sanguis draxonis on spared nerve injury rat model of neuropathic pain

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• 作者：Fu-Feng Chen^a ; Fu-Quan Huo^b ; ¹ ; Hui Xiong^a ; ^{huofq@mail.xjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Qing Wan^a ; Ya-Nan Zheng^a ; Wen-Jie Du^a ; Zhi-Nan Mei^a ; ¹ ; ^{meizhinan@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Total flavonoids from Sanguis draxonis ; Spared nerve injury ; Neuropathic pain ; Astrocyte ; Pro-inflammatory cytokines
• 刊名：Phytomedicine
• 出版年：2015
• 出版时间：15 November 2015
• 年：2015
• 卷：22
• 期：12
• 页码：1125-1132
• 全文大小：2529 K

文摘

Sanguis draxonis (SD) is a kind of red resin obtained from the wood of Dracaena cochinchinensis (Lour.) S. C. Chen (D. cochinchinensis). The active components of total flavonoids from SD (SDF) have analgesic effect.

Aim

The aim of this study is to evaluate the analgesic effects and potential mechanism of SDF on mechanical hypersensitivity induced by spared nerve injury (SNI) model of neuropathic pain in the rat.

Methods

SNI model in rats was established and then the rats were treated with SDF intragastric administration for 14 days. Paw withdrawal mechanical threshold (PMWT) in response to mechanical stimulation was measured by von Frey filaments on day 1 before operation and days 1, 3, 5, 7, 9, 11, 14 after operation, respectively. After 14 days, we measured the levels of nitric oxide (NO), nitric oxide synthase (NOS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-10 (IL-10) in the spinal dorsal horn. In addition, the expression of fibroblast growth factor receptor 3 (FGFR3), phosphorylated cyclic AMP response element-binding protein (p-CREB) and glial fibrillary acidic protein (GFAP) of the spinal dorsal horn was evaluated by western blotting and an immunofluorescence histochemical method, respectively.

Results

Intragastric administration of SDF (100, 200, 400 mg/kg) alleviated significantly SNI-induced mechanical hypersensitivity, as PMWT increased in a dose-dependent manner. Moreover, SDF not only reduced the level of NO, NOS, TNF-α and IL-1β, but also upregulated the level of IL-10 in the spinal dorsal horn of SNI rats. At the same time, SDF (100, 200, 400 mg/kg) could inhibit the expression of FGFR3, GFAP and p-CREB in the spinal dorsal horn.

Conclusion

SDF has potentially reduced mechanical hypersensitivity induced by SNI model of neuropathic pain which may be attributed to inhibition of astrocytic function (like release pro-inflammatory cytokines) and NO release as well as p-CREB activation in the spinal dorsal horn.