Enhanced antisense effect of modified PNAs delivered through functional PMMA microspheres
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- 作者：Laura Chiarantini ; Aurora Cerasi ; Enrico Millo ; Katia Sparnacci ; Michele Laus ; Massimo Riccio ; Spartaco Santi ; Marco Ballestri ; Silvia Spaccasassi and Luisa Tondelli
- 关键词：Peptide nucleic acids (PNA) ; Core-shell microspheres ; Ciclooxigenase-2 (COX-2) ; PMMA microspheres ; Macrophages ; Antisense therapy
- 刊名：International Journal of Pharmaceutics
- 出版年：2006
- 出版时间：31 October 2006
- 年：2006
- 卷：324
- 期：1
- 页码：83-91
- 全文大小：734 K
文摘
Peptide nucleic acids (PNA) are very promising antisense agents, but their in vivo application is often hampered by their low bioavailability, mainly due to their limited uptake through cellular and nuclear membranes. However, PNA chemical synthesis easily allows modification with functional structures able to improve the intrinsically low permeability and great interest is arising in finding specific and efficient delivery protocols. Polymeric core-shell microspheres with anionic functional groups on the surface were tested for their ability to reversibly bind lysine modified PNA sequences, whose antisense activity against COX-2 mRNA was already demonstrated in murine macrophages.