DNA prime and protein boost immunization with innovative polymeric cationic core-shell nanoparticles elicits broad immune responses and strongly enhance cellular responses of HIV-1 tat DNA vaccination
详细信息 查看全文
- 作者：Arianna Castaldello ; Egidio Brocca-Cofano ; Rebecca Voltan ; Chiara Triulzi ; Giuseppe Altavilla ; Michele Laus ; Katia Sparnacci ; Marco Ballestri ; Luisa Tondelli ; Cinzia Fortini et al.
- 关键词：Biocompatible nanoparticles ; HIV-1 tat ; DNA vaccination ; Cellular responses
- 刊名：Vaccine
- 出版年：2006
- 出版时间：17 July 2006
- 年：2006
- 卷：24
- 期：29-30
- 页码：5655-5669
- 全文大小：729 K
文摘
Novel biocompatible core-shell cationic nanoparticles, composed of an inner hard core of poly(methylmethacrylate) (PMMA) and a hydrophilic tentacular shell bearing positively charged groups and poly(ethyleneglycol) chains covalently bound to the core, were prepared by emulsion polymerization and characterized in vitro and in vivo for DNA vaccine applications. The nanoparticles reversibly adsorbed large amounts of DNA, mainly through electrostatic interactions, preserved its functional structure, efficiently delivered it intracellularly, and were not toxic in vitro or in mice. Furthermore, two intramuscular (i.m.) immunizations (4 weeks apart) with a very low dose (1 μg) of the plasmid pCV-tat delivered by these nanoparticles followed by one or two protein boosts induced significant antigen-specific humoral and cellular responses and greatly increased Th1-type T cell responses and CTLs against HIV-1 Tat.