Measured samples: diesel, rapeseed and soybean oil methyl esters (pure and blends). Density and viscosity of biofuels have been measured up to 300 MPa. Results are obtained for temperature up to 60 °C to study cold start conditions. Heat capacity has been calculated by literature density and speed of sound results. Freezing phenomena were observed for temperature up to 30 °C from 135 MPa.