Evaluation of rapid GeneXpert MTB/RIF method using DNA tissue specimens of vertebral bones in patients with suspected spondylitis TB

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• 作者：Muhammad Nasrum Massi^a ; ^{nasrumm2000@yahoo.com} ; Karya Triko Biatko^b ; Irda Handayani^c ; Muhammad Yogi Pratama^a ; Sari Septriani^d ; Gaby Maulida Nurdin^d ; Marina B. Ali^d
• 关键词：DNA M. tuberculosis ; GeneXpert MTB/RIF ; Specimen pus ; Spondylitis TB
• 刊名：Journal of Orthopaedics
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：14
• 期：1
• 页码：189-191
• 全文大小：219 K
• 卷排序：14

文摘

To detect Mycobacterium tuberculosis DNA and rifampicin resistance in vertebral bone tissue specimens from spondylitis TB suspects.Methods and resultsThe rapid GeneXpert MTB/RIF and MGIT 960 liquid culture methods have been used in the specimens. Results from 70 suspects with spondylitis TB shown that 31.42% identified positive for spondylitis TB using culture method, while 88.57% shown positive results using rapid GeneXpert MTB/RIF method. The validity of GeneXpert MTB/RIF shown sensitivity value of 100%, specificity value of 16.6%, PPV of 35.48%, and NPV of 100%.ConclusionGeneXpert has a high sensitivity but low specificity value in this study.