文摘
A breakthrough of the simultaneous quantification of major metabolites from herb medicines was achieved without standard compounds using online mircrodialysis-ultra performance liquid chromatography-mass spectrometry. It is the first time to investigate the compare pharmacokinetics of two major metabolites, namely, genipin-1-o-glucuronic acid and genipin-monosulfate from geniposide and genipin between healthy and diabetic rats by online MD-UPLC–MS/MS. The results showed that their pharmacokinetic behaviors in type 2 diabetic rats significantly differ from healthy rats (p < 0.05). The approach could predict druggability of bioactive metabolites from their prototypes.