A comprehensive profiling of sulfatides in myelin from mouse brain using liquid chromatography coupled to high-resolution accurate tandem mass spectrometry

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• 作者：M. Pintado-Sierra^a ; I. Garcí ; a-Á ; lvarez^a ; ^b ; A. Bribiá ; n^b ; ^c ; E.M. Medina-Rodrí ; guez^b ; R. Lebró ; n-Aguilar^d ; L. Garrido^e ; F. de Castro^b ; ^c ; A. Ferná ; ndez-Mayoralas^a ; J.E. Quintanilla-Ló ; pez^d ; ^{je.quintanilla@iqfr.csic.es}
• 关键词：Sulfatides ; Myelin ; Mouse brain ; Liquid chromatography-tandem mass spectrometry ; Accurate mass
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：25 January 2017
• 年：2017
• 卷：951
• 期：Complete
• 页码：89-98
• 全文大小：751 K
• 卷排序：951

文摘

Thirty seven sulfatides have been simultaneously determined in myelin by LC-ESI-MS/MS. Wrong-way-round ionization effect allowed to obtain very good sensitivity in ESI(+). MS/MS fragmentation pattern allowed differentiating structural isomers. High mass accuracy was necessary to get a reliable identification of sulfatides. Sulfatides with hydroxylated and/or odd numbered fatty acid chains increase with age.