A comprehensive profiling of sulfatides in myelin from mouse brain using liquid chromatography coupled to high-resolution accurate tandem mass spectrometry
Thirty seven sulfatides have been simultaneously determined in myelin by LC-ESI-MS/MS. Wrong-way-round ionization effect allowed to obtain very good sensitivity in ESI(+). MS/MS fragmentation pattern allowed differentiating structural isomers. High mass accuracy was necessary to get a reliable identification of sulfatides. Sulfatides with hydroxylated and/or odd numbered fatty acid chains increase with age.