An efficient and high fidelity method for amplification, cloning and sequencing of complete tospovirus genomic RNA segments

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• 作者：Spencer H. Marshall^a ; ¹ ; Raphael O. Adegbola^a ; ¹ ; Scott Adkins^b ; Rayapati A. Naidu^a ; ^{naidu.rayapati@wsu.edu}
• 关键词：Bunyaviridae ; Tospovirus ; Tomato spotted wilt virus ; Impatiens necrotic spot virus ; M-RNA ; S-RNA
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：22-26
• 全文大小：639 K
• 卷排序：242

文摘

Methods optimized for amplification of complete M-and S-RNA genome segments of TSWV and INSV as single fragments. Advantageous for whole genome sequencingof tospoviruses compared to overlapping amplicon-basedapproaches. Useful tool for investigating epidemiology and evolution of tospoviruses in mixed infections within natural hosts.