Effect of a regulatory mutation on the rat atrial natriuretic peptide gene transcription

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• 作者：Rubattu ; Speranza ; Giliberti ; Rosangela ; De Paolis ; Paola ; Stanzione ; Rosita ; Spinsanti ; Paola ; et. al.
• 关键词：Atrial natriuretic peptide ; ANP promoter ; PEA2 ; Gene mutation
• 刊名：Peptides
• 出版年：2002
• 出版时间：March, 2002
• 年：2002
• 卷：23
• 期：3
• 页码：555-560
• 全文大小：109 K

文摘

To investigate the functional relevance of a regulatory mutation affecting the enhancer element PEA2 of the rat ANP gene we transfected rat cardiomyocytes and aortic endothelial cells with either the mutant or the wild-type ANP promoter construct (-683 +54) and performed CAT assays both at baseline and in response to Phenylephrine and Angiotensin II. In the myocardial cells we also determined the DNA/nuclear protein interaction through electrophoretic mobility shift assay. These studies showed a significantly lower degree of ANP transcription in the presence of the mutant PEA2 site, thus demonstrating its functional significance and the biological relevance of ANP gene structural alterations.