An improved non-denaturing method for the purification of spiralin, the main membrane lipoprotein of the pathogenic bacteria Spiroplasma melliferum
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- 作者：Yann Desfougè ; resa ; y.desfougeres@ucl.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Jean-Michel Poitoub ; 1 ; Henri Wró ; blewskia ; Laure Bé ; vena ; b ; 2
- 关键词：CE-HPLC ; Detergents ; Membrane lipoprotein ; Spiralin ; Spiroplasma melliferum ; Mollicutes
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：1036-1037
- 期：Complete
- 页码：149-156
- 全文大小：1464 K
文摘
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An improved procedure for the purification of a bacterial lipoprotein is presented.
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The new procedure shortens the purification from 24 h to 4 h.
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Detergent screening revealed that spiralin is mostly insoluble in Sarkosyl and Triton X-100.
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Milligram quantities of highly pure spiralin can be obtained.
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The procedure is non-denaturing.