Carrier-bound, nonallergenic Ole e 1 peptides for vaccination against olive pollen allergy

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• 作者：Teresa E. Twaroch MSc^a ; Margit Focke PhD^a ; Vera Civaj^a ; Milena Weber MSc^a ; Nadja Balic^b ; Adriano Mari MD^c ; Rosetta Ferrara MD^c ; Santiago Quirce MD^d ; Susanne Spitzauer MD^b ; Ines Swoboda PhD^a ; ^{ines.swoboda@meduniwien.ac.at" rel="nofollow} ; Rudolf Valenta MD^a
• 关键词：Olive pollen allergy ; Ole e 1 ; specific immunotherapy ; peptides ; epitopes
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：128
• 期：1
• 页码：178-184.e7
• 全文大小：639 K

文摘

Background

Trees of the family Oleaceae (olive and ash) are important allergen sources in Mediterranean countries, Northern and Central Europe, and North America. The major olive pollen allergen Ole e 1 represents the majority of allergenic epitopes in olive pollen and cross-reacts with Fra e 1, the major ash pollen allergen.

Objective

We sought to develop a safe vaccine for the treatment of Oleaceae pollen allergy.

Methods

We synthesized 5 peptides ranging from 32 to 36 amino acids, which covered the whole sequence of Ole e 1. The IgE and T-cell reactivity of the peptides was compared with that of Ole e 1 by means of dot blot experiments, as well as ELISA, and in proliferation assays. Rabbits were immunized with non–IgE-reactive, keyhole limpet hemocyanin–coupled peptides or Ole e 1. The reactivity of the IgG antibodies with Ole e 1 and their ability to inhibit IgE binding to nOle e 1 was evaluated by means of ELISA.

Results

Only the C-terminal Ole e 1 peptide showed IgE binding, whereas the other peptides were nonallergenic. Immunization of rabbits with Ole e 1–derived peptides bound to the carrier molecule keyhole limpet hemocyanin induced in rabbits the production of Ole e 1–specific IgG antibodies, which cross-reacted with Fra e 1, and inhibited olive and ash pollen–sensitized patients' IgE binding to Ole e 1.

Conclusion

Two non–IgE-binding peptides with low T-cell reactivity from the N-terminus of Ole e 1 were identified that might represent safe vaccine candidates for immunotherapy of Oleaceae pollen allergy.