Staphylococcus aureus fibronectin-binding protein specifically binds IgE from patients with atopic dermatitis and requires antigen presentation for cellular immune responses

详细信息    查看全文

• 作者：Kavita Reginald PhD^a ; Kerstin Westritschnig MD^a ; Birgit Linhart PhD^a ; Margarete Focke-Tejkl PhD^b ; Beatrice Jahn-Schmid PhD^a ; Julia Eckl-Dorna MD ; PhD^c ; Annice Heratizadeh MD^f ; Angelika Stö ; cklinger PhD^g ; Nadja Balic^d ; Susanne Spitzauer MD^d ; Verena Niederberger MD^c ; Thomas Werfel MD^f ; Josef Thalhamer PhD^g ; Stephan Weidinger MDⁱ ; Natalija Novak MD^j ; Markus Ollert MD^h ; ⁱ ; Alexander M. Hirschl MD^e ; Rudolf Valenta MD^a ; ^b ; ^{rudolf.valenta@meduniwien.ac.at"" rel=""nofollow}
• 关键词：Staphylococcus aureus ; atopic dermatitis ; bacterial antigen ; bacterial allergen ; allergy ; IgE ; superinfection ; superantigen
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：128
• 期：1
• 页码：82-91.e8
• 全文大小：1477 K

文摘

Background

Staphylococcus aureus superinfections occur in more than 90 % of patients with atopic dermatitis (AD) and aggravate skin inflammation. S aureus toxins lead to tissue damage and augment T-cell–mediated skin inflammation by a superantigen effect.

Objective

To characterize IgE-reactive proteins from S aureus.

Methods

A genomic S aureus library was screened with IgE from patients with AD for DNA clones coding for IgE-reactive antigens. One was identified as fibronectin-binding protein (FBP). Recombinant FBP was expressed in Escherichia coli, purified, and tested for specific IgE reactivity in patients with AD. Its allergenic activity was studied in basophil activation experiments and T-cell cultures. The in vivo allergenic activity was investigated by sensitizing mice.

Results

Using IgE from patients with AD for screening of a genomic S aureus library, an IgE-reactive DNA clone was isolated that coded for FBP. Recombinant FBP was expressed in E coli and purified. It reacted specifically with IgE from patients with AD and exhibited allergenic activity in basophil degranulation assays. FBP showed specific T-cell reactivity requiring antigen presentation and induced the secretion of proinflammatory cytokines from PBMCs. Mice sensitized with FBP mounted FBP-specific IgE responses, showed FBP-specific basophil degranulation as well as FBP-specific T-cell proliferation, and mixed T_h2/T_h1 cytokine secretion.

Conclusion

Evidence is provided that specific humoral and cellular immune responses to S aureus antigens dependent on antigen presentation represent a novel mechanism for S aureus–induced skin inflammation in AD. Furthermore, FBP may be used for the development of novel diagnostic and therapeutic strategies for S aureus infections.