Nucleotide sequence of phospholipase A₂ gene expressed in snake pancreas reveals the molecular evolution of toxic phospholipase A₂ genes

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• 作者：Fujimi ; Takahiko J. ; Kariya ; Yoshinobu ; Tsuchiya ; Takahide ; Tamiya ; Toru
• 关键词：Phospholipase A₂ ; Gene structure ; Pancreas loop ; Molecular evolution ; CR1 ; chicken repeat 1 ; LINE ; long interspersed repeated DNA ; LsPLA2 ; Laticauda semifasciata phospholipase A₂ ; PLA₂ ; phospholipase A₂
• 刊名：Gene
• 出版年：2002
• 出版时间：June 12, 2002
• 年：2002
• 卷：292
• 期：1-2
• 页码：225-231
• 全文大小：388 K

文摘

We have cloned two phospholipase A₂ (PLA₂) DNA complementary to RNA that contained nucleotide sequences encoding pancreas loop by the reverse transcription-polymerase chain reaction cloning procedure using messenger RNA isolated from Laticauda semifasciata pancreas. Additionally, a gene clone encoding PLA₂ with the pancreatic loop sequence was isolated from a L. semifasciata genomic library. Subsequent sequence analysis revealed that PLA₂ clones encoding group IB″ PLA₂. Comparative analysis of group IA and IB″ PLA₂ genes revealed that the exon-intron organization is conserved in the genes of both groups. The invaded sequences in the second intron were very similar to those of the L. semifasciata group IA gene. This observation suggested that the integration of the invaded sequences occurred before the divergence of groups IA and IB″ during the evolution of PLA₂ gene. The comparative analysis revealed that the arising of group IA PLA₂ occurred by the deletion and substitution of nucleotide sequences in exon III region during the process of accelerated evolution.