Glutathione transferase (GST) as a candidate molecular-based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus

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• 作者：E. James LaCourse ; Mariluz Hern ; ez-Viadel ; James R. Jefferies ; Claus Svendsen ; David J. Spurgeon ; John Barrett ; A. John Morgan ; Peter Kille ; Peter M. Brophy
• 关键词：Glutathione transferase ; Lumbricus rubellus ; Earthworm ; 2-DE
• 刊名：Environmental Pollution
• 出版年：2009
• 出版时间：August-September 2009
• 年：2009
• 卷：157
• 期：8-9
• 页码：2459-2469
• 全文大小：703 K

文摘

The earthworm Lumbricus rubellus (Hoffmeister, 1843) is a terrestrial pollution sentinel. Enzyme activity and transcription of phase II detoxification superfamily glutathione transferases (GST) is known to respond in earthworms after soil toxin exposure, suggesting GST as a candidate molecular-based pollution biomarker. This study combined sub-proteomics, bioinformatics and biochemical assay to characterise the L. rubellus GST complement as pre-requisite to initialise assessment of the applicability of GST as a biomarker. L. rubellus possesses a range of GSTs related to known classes, with evidence of tissue-specific synthesis. Two affinity-purified GSTs dominating GST protein synthesis (Sigma and Pi class) were cloned, expressed and characterised for enzyme activity with various substrates. Electrospray ionisation mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS) following SDS-PAGE were superior in retaining subunit stability relative to two-dimensional gel electrophoresis (2-DE). This study provides greater understanding of Phase II detoxification GST superfamily status of an important environmental pollution sentinel organism.