Assessment of denitrifying bacterial composition in activated sludge

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• 作者：C.S.  ; Srinandan ; Mrinal  ; Shah ; Bhavita  ; Patel ; Anuradha S.  ; Nerurkar  ; ;   ; ^{anuner26@yahoo.com}
• 关键词：Denitrifying bacterial community ; Activated sludge ; Denitrification
• 刊名：Bioresource Technology
• 出版年：2011
• 出版时间：October 2011
• 年：2011
• 卷：102
• 期：20
• 页码：9481-9489
• 全文大小：789 K

文摘

The abundance and structure of denitrifying bacterial community in different activated sludge samples were assessed, where the abundance of denitrifying functional genes showed nirS in the range of 10⁴?0⁵, nosZ with 10⁴?0⁶ and 16S rRNA gene in the range 10⁹?0¹⁰ copy number per ml of sludge. The culturable approach revealed Pseudomonas sp. and Alcaligenes sp. to be numerically high, whereas culture independent method showed betaproteobacteria to dominate the sludge samples. Comamonas sp. and Pseudomonas fluorescens isolates showed efficient denitrification, while Pseudomonas mendocina, Pseudomonas stutzeri and Brevundimonas diminuta accumulated nitrite during denitrification. Numerically dominant RFLP OTUs of the nosZ gene from the fertilizer factory sludge samples clustered with the known isolates of betaproteobacteria. The data also suggests the presence of different truncated denitrifiers with high numbers in sludge habitat.