Ultra-high sensitivity analysis of estrogens for special populations in serum and plasma by liquid chromatography-mass spectrometry: Assay considerations and suggested practices
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- 作者：Qingqing Wanga ; b ; c ; Clementina Mesarosa ; b ; Ian A. Blaira ; b ; ianblair@mail.med.upenn.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Estrogens and their metabolites ; Older men ; Children ; Postmenopausal women ; Liquid chromatograph&ndash ; tandem mass spectrometry ; Stable isotope dilution methodology ; Derivatization
- 刊名：Journal of Steroid Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：162
- 期：Complete
- 页码：70-79
- 全文大小：2225 K
文摘
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It remains an analytical challenge to quantify estrogens and their metabolites in specimens from special populations.
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Estrogen levels are at pg/mL in serum or plasma samples from older men, children, postmenopausal women and women receiving aromatase inhibitors for breast cancer treatment.
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Stable isotope dilution LC-SRM/MS assays provide high specificity and accuracy.
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Estrogen derivatives facilitate ultra-high sensitivity LC-SRM/MS-based analysis.
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Suggested practices for ultra-high sensitivity LC-SRM/MS-based methodology are reviewed
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Future perspectives on the use of high-resolution mass spectrometry are discussed.