Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP
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- 作者：Yu Koarashia ; b ; 1 ; Abraham G. Cá ; ceresc ; d ; 1 ; Florencia Margarita Zú ; niga Sacae ; Elsa Elvira Palacios Floresf ; Adela Celis Trujillof ; José ; Luis Abanto Alvaresg ; Kumiko Yoshimatsub ; Jiro Arikawab ; Ken Katakuraa ; Yoshihisa Hashiguchih ; i ; Hirotomo Katoa ; hkato@vetmed.hokudai.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Leishmania ; Mannose phosphate isomerase ; PCR-RFLP ; Peru
- 刊名：Acta Tropica
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：158
- 期：Complete
- 页码：83-87
- 全文大小：636 K
文摘
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A PCR-RFLP was established to differentiate Leishmania species in Huanuco, Peru.
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The technique was applied to 267 DNA samples from patients with cutaneous leishmaniasis.
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All the PCR-amplified samples were successfully identified at the species level.
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The technique will be a useful tool for epidemiological study of leishmaniasis.