Optimising Golgi-Cox staining for use with perfusion-fixed brain tissue validated in the zQ175 mouse model of Huntington's disease
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- 作者：Zubeyde Bayram-Weston ; Bayram-WestonZ@cardiff.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Elliott Olsen ; David J. Harrison ; Stephen B. Dunnett ; Simon P. Brooks
- 关键词：Golgi&ndash ; Cox method ; Paraformaldehyde ; Section thickness ; Huntington's disease ; zQ175 mice
- 刊名：Journal of Neuroscience Methods
- 出版年：2016
- 出版时间：30 May 2016
- 年：2016
- 卷：265
- 期：Complete
- 页码：81-88
- 全文大小：1222 K
文摘
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A modified reliable Golgi–Cox method is described.
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It is inexpensive and can be performed in any neuroscience lab.
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Perfusion–fixation with 4% paraformaldehyde yields good results.
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Pre-sectioning of perfusion-fixed brain tissue is neither required nor preferred for modified Golgi–Cox staining.
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We demonstrate reliable changes in striatal dendritic morphology in the Q175 mouse model of Huntington's disease.