Membrane damage by betulinic acid provides insights into cellular aging

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• 作者：Waleska K. Martins^a ; ^b ; Andreza B. Gomide^c ; ^d ; É ; rico T. Costa^e ; Helena C. Junqueira^a ; Beatriz S. Stolf^f ; Rosangela Itri^c ; Maurí ; cio S. Baptista^a ; ^{baptista@iq.usp.br}
• 关键词：AAU ; Autophagy Arbitrary Units ; BA ; Betulinic Acid ; Cathepsin B ; CTSB ; CQ ; Chloroquine ; COXIV ; cytochrome c oxidase subunit iv ; CVS ; Crystal Violet Staining ; DMEM ; Dulbecco's modified Eagle's medium ; DDR ; DNA damage response ; FBS ; fetal bovine serum ; GAPDH ; Glyceraldehyde-3-Phosphate Dehydrogenase ; LMP ; Lysosomal membrane permeabilization ; MTT ; 3-(4 ; 5-Dimethylthiazol-2-yl)-2 ; 5-Diphenyltetrazolium Bromide ; NR ; Neutral Red ; OIS ; oncogene-induced senescence ; POPC ; 1-palmitoyl ; 2-oleoyl-sn-glycero-3-
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1861
• 期：1
• 页码：3129-3143
• 全文大小：2052 K
• 卷排序：1861

文摘

The mechanistic trigger of cell senescence from a condition of autophagy deficiency is proposed. Betulinic Acid (BA) physically interacts and disorganizes membranes, causing membrane leakage in membrane mimics and in intracellular organelles. Parallel damage in the membranes of mitochondria and lysosome causes lipofuscinogenesis, genomic instability and cell senescence. Senescence is the long-term consequence of treating cells with BA.