Use of short-end injection capillary packed with a glycopeptide antibiotic stationary phase in electrochromatography and capillary liquid chromatography for the enantiomeric separation of hydroxy acid

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• 作者：Fanali ; Salvatore ; Catarcini ; Paolo ; Presutti ; Carla ; Stancanelli ; Rosanna ; Quaglia ; M.G.
• 关键词：Injection methods ; Chiral stationary phases ; electrochromatography ; Chiral stationary phases ; LC ; Electrochromatography ; Glycopeptide ; Peptides ; Antibiotics ; Hydroxy acids ; Teicoplanin ; Mandelic acid
• 刊名：Journal of Chromatography A
• 出版年：2003
• 出版时间：March 21, 2003
• 年：2003
• 卷：990
• 期：1-2
• 页码：143-151
• 全文大小：366 K

文摘

A new chiral stationary phase (CSP) was prepared by reacting MDL 63,246 (Hepta-Tyr), a glycopeptide antibiotic belonging to the teicoplanin family, with 5-μm diol-silica particles. The CSP mixed with 5-μm amino silica particles (3:1) was packed into 75-μm fused-silica capillaries for only 6.6 cm and used for electrochromatographic experiments analyzing several hydroxy acid enantiomers. A reversed electroosmotic flow carried both analytes and mobile phase towards the anode in a short time (1–3 min), being baseline resolved all the studied analytes. In order to achieve the fastest enantiomeric resolution of the studied hydroxy acids, the effect of several experimental parameters such as mobile phase composition (organic modifier type and concentration, pH of the buffer and ionic strength), capillary temperature and applied voltage on enantioresolution factor, retention time, enantioselectivity were evaluated. The packed capillary column allowed the separation of mandelic acid enantiomers in less than 72 s with resolution factor R_s