A characterization study of resveratrol/sulfobutyl ether-尾-cyclodextrin inclusion complex and in vitro anticancer activity

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• 作者：Valentina Venuti^a ; Carmela Cannavà ; ^b ; Maria Chiara Cristiano^c ; Massimo Fresta^c ; Domenico Majolino^a ; Donatella Paolino^c ; Rosanna Stancanelli^b ; Silvana Tommasini^b ; Cinzia Anna Ventura^b ; ^{caventura@unime.it" class="auth_mail}
• 关键词：Resveratrol ; Sulfobutylether-尾-cyclodextrin ; FTIR-ATR ; Job's plot ; Anticancer activity
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：1 March, 2014
• 年：2014
• 卷：115
• 期：Complete
• 页码：22-28
• 全文大小：712 K

文摘

A resveratrol/sulfobutylether-尾-cyclodextrin inclusion complex was prepared using the freeze-drying method and characterized in solution through UV-vis spectroscopy, solubility phase studies and Job's plot methods. At the solid state it was characterized using the FTIR-ATR technique. Sulfobutylether-尾-cyclodextrin has a high affinity for the drug, and forms an inclusion complex with a 1:1 molar ratio both in solution and as a solid sample. It also has a high stability constant (K_c, 10,114 M^鈭?). Complexation strongly increases the water solubility of resveratrol (from 0.03 mg/ml to 1.1 mg/ml, at 25 掳C) and positively influences its in vitro anticancer activity which was observed on a human breast cancer cell line (MCF-7). In solid phase, FTIR-ATR revealed itself as being a useful technique in elucidating the complexation mechanism, which it did by emphasizing the functional groups involved in the activation of non-covalent 鈥渉ost-guest鈥?interactions.