Oxidative stress responses in zebrafish Danio rerio after subchronic exposure to atrazine
详细信息 查看全文
- 作者：Jana Blahov谩 ; Lucie Plhalov谩 ; Martin Hostovsk媒 ; Lenka Divi拧ov谩 ; Radka Dob拧铆kov谩 ; Ivana Mikul铆kov谩 ; Stanislava 艩t臎p谩nov谩 ; Zde艌ka Svobodov谩
- 关键词：CAT ; catalase ; GPx ; glutathione peroxidase ; GR ; glutathione reductase ; GST ; glutathione S-transferase ; NBT ; nitroblue tetrazolium ; r ; specific growth rate ; ROS ; reactive oxygen species ; SEM ; standard error of mean ; SOD ; superoxide dismutase ; TBARS ; thiobarbituric acid reactive substances
- 刊名：Food and Chemical Toxicology
- 出版年：November, 2013
- 年：2013
- 卷：61
- 期：Complete
- 页码：82-85
- 全文大小：243 K
文摘
Atrazine is one of the most used pesticides all over the world and it is frequently detected in surface water. The aim of this study was to investigate if zebrafish exposure to atrazine could induce oxidative stress and changes in detoxifying system. Juvenile fish were exposed to sublethal concentrations of 0.3, 3, 30, or 90 渭g Lp>鈭? p> for 28 days. The level of oxidized lipids increased in experimental groups exposed to atrazine at 30 and 90 渭g Lp>鈭? p> compared to control. Activity of glutathione S-transferase decreased in group with the highest concentration compared to control. A significant decline was observed in catalase activity in all experimental groups compared to control. Activity of superoxide dismutase increased only in experimental group exposed to atrazine at 30 渭g Lp>鈭? p> compared to control. Activity of glutathione peroxidase and reductase (GR) increased in experimental groups exposed to atrazine at 0.3 (only for GR activity) and 90 渭g Lp>鈭? p> compared to control. Our results showed that atrazine exposure had profound influence on the oxidative stress markers and detoxifying enzyme of the exposed zebrafish. The changes in antioxidant enzyme activities could be an adaptive response to protect the fish from the atrazine-induced toxicity.