Superparamagnetic iron oxide-encapsulating polymersome nanocarriers for biofilm eradication

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• 作者：Benjamin M. Geilich^a ; ^b ; Ilia Gelfat^c ; Srinivas Sridhar^b ; ^d ; Anne L. van de Ven^b ; ^d ; Thomas J. Webster^c ; ^e ; ^{th.webster@northeastern.edu}
• 关键词：Biofilm ; Polymersome ; SPION ; Staphylococcus epidermidis ; Antibiotic-resistance ; Nanomedicine
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：119
• 期：Complete
• 页码：78-85
• 全文大小：3064 K
• 卷排序：119

文摘

The rising prevalence and severity of antibiotic-resistant biofilm infections poses an alarming threat to public health worldwide. Here, biocompatible multi-compartment nanocarriers were synthesized to contain both hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) and the hydrophilic antibiotic methicillin for the treatment of medical device-associated infections. SPION co-encapsulation was found to confer unique properties, enhancing both nanocarrier relaxivity and magneticity compared to individual SPIONs. These iron oxide-encapsulating polymersomes (IOPs) penetrated 20 μm thick Staphylococcus epidermidis biofilms with high efficiency following the application of an external magnetic field. Three-dimensional laser scanning confocal microscopy revealed differential bacteria death as a function of drug and SPION loading. Complete eradication of all bacteria throughout the biofilm thickness was achieved using an optimized IOP formulation containing 40 μg/mL SPION and 20 μg/mL of methicillin. Importantly, this formulation was selectively toxic towards methicillin-resistant biofilm cells but not towards mammalian cells. These novel iron oxide-encapsulating polymersomes demonstrate that it is possible to overcome antibiotic-resistant biofilms by controlling the positioning of nanocarriers containing two or more therapeutics.