Interrogation of single nucleotide polymorphisms in gnd provides a novel method for molecular serogrouping of clinically important Shiga toxin producing Escherichia coli (STEC) targeted by regulation in the United States, including the “big six” non-O157 STEC and STEC O157
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- 作者：J.R. Eldera ; b ; M. Bugarela ; H.C. den Bakkera ; c ; G.H. Loneragana ; K.K. Nightingalea ; b ; kendra.nightingale@ttu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Shiga toxin producing Escherichia coli (STEC) ; E. coli O157 ; H7 ; Non-O157 STEC ; Molecular serotyping ; SNP typing ; gnd
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：129
- 期：Complete
- 页码：85-93
- 全文大小：292 K
文摘
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An assay was developed to group shiga toxin producing Escherichia coli isolates into clinically important serogroups regulated in U.S.
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The multiplex SNP typing assay developed here interrogates 12 informative SNPs in gnd.
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Each regulated STEC serogroup contained multiple gnd SNP types distinct from SNP genotypes belonging to other serogroups.
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The assay represents a rapid high-throughput alternative to conventional serogrouping for surveillance and epidemiological investigations.