Quantification of urinary PGEm, 6-keto PGF_1α and 2,3-dinor-6-keto PGF_1α by UFLC–MS/MS before and after exercise

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• 作者：Matthew Blatnik ; Rick C. Steenwyk
• 关键词：2 ; 3-Dinor-6-keto PGF_1α ; ; 6-Keto PFG_1α ; ; Eicosanoids ; ESI ; Electrospray ionization ; Exercise ; Inflammation ; MS/MS ; Tandem mass spectrometry ; PGEm ; Prostanoids ; UFLC ; Ultra-flow liquid chromatography
• 刊名：Prostaglandins and Other Lipid Mediators
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：93
• 期：1-2
• 页码：8-13
• 全文大小：264 K

文摘

Eicosanoids play an important role in the evaluation of pro-inflammatory responses and in the safety and toxicity of novel therapeutic agents. This work describes a high-throughput UFLCMS/MS method for the analysis of three urinary prostanoid biomarkers of pro-inflammatory responses, tetranor PGEm, 6-keto PGF_1α and 2,3-dinor-6-keto PFG_1α. Nine male volunteers of various age and fitness level participated in this study. Six provided pre- and post-exercise samples and three provided intraday samples. Tetranor PGEm and 6-keto PGF_1α increased significantly in patients after exercise (p < 0.017 and p < 0.029). In individual patient sets, tetranor PGEm levels increased from 1.5- to 6-fold pre- vs. post-exercise, levels of 6-keto PGF_1α increased more dramatically from 2- to 55-fold pre- vs. post-exercise. The prostanoid 2,3-dinor-6-keto PGF_1α remained unchanged post-exercise. Data was normalized to urinary creatinine concentration, which increased approximately 40 % post-exercise.