Linkage between C-reactive protein and triglyceride-rich lipoprotein metabolism

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• 作者：Nuntakorn Thongtang^a ; ^b ; ^{nuntakorn.thongtang@tufts.edu} ; Margaret R. Diffenderfer^a ; Esther M.M. Ooi^a ; ^c ; Bela F. Asztalos^a ; Gregory G. Dolnikowski^a ; Stefania Lamon-Fava^a ; Ernst J. Schaefer^a
• 关键词：CRP ; C-reactive protein ; PR ; production rate ; FCR ; fractional catabolic rate ; PS ; pool size ; TRL ; triglyceride-rich lipoprotein ; apoB ; apolipoprotein B ; apoA-I ; apolipoprotein A-I
• 刊名：Metabolism
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：62
• 期：3
• 页码：369-375
• 全文大小：507 K

文摘

Objective

Inflammation plays an important role in atherosclerosis. Elevated C-reactive protein (CRP) levels are associated with a greater risk of cardiovascular disease. Our goal was to study CRP metabolism, and to determine its relationship with lipoprotein metabolism using stable isotope methodology.

Material/Methods

Eight subjects with combined hyperlipidemia underwent a 15-h primed-constant infusion with deuterated leucine. CRP was purified from the plasma density fraction greater than 1.21 g/ml by affinity chromatography. Lipoprotein fractions were separated by sequential ultracentrifugation. Isotope enrichment was determined by gas chromatography/mass spectrometry.

Results

The subjects had mean LDL-C levels of 147.5 mg/dl and mean CRP levels of 3.4 mg/l. The mean CRP production rate (PR) was 0.050 ¡À 0.012 mg/kg/day and the mean CRP fractional catabolic rate (FCR) was 0.343 ¡À 0.056 pools/day (residence time 2.92 days). CRP pool size (PS) was significantly related to production (r = 0.93; p < 0.001), but not FCR. CRP PS was also related to body mass index (r = 0.79; p = 0.02). There was a significant association between CRP FCR and TRL apoB-100 FCR (r = 0.74, p = 0.04), as well as between CRP PS and TRL apoB-48 FCR (r = ? 0.90, p = 0.002), indicating linkage between CRP and TRL metabolism.

Conclusion

The main determinant of plasma CRP levels was CRP production rate. Moreover a significant linkage between CRP metabolism and both TRL apoB-100 and apoB-48 catabolism was noted.