Using the plant vacuole as a biological system to investigate the functional properties of exogenous channels and transporters
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- 作者：M. Festa1 ; L. Lagostena1 ; A. Carpaneto ; carpaneto@ge.ibf.cnr.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Plant vacuole ; Lipodepsipeptide ; Patch-clamp ; Intracellular channels ; TPC channels
- 刊名：Biochimica et Biophysica Acta (BBA)-Biomembranes
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：1858
- 期：3
- 页码：607-612
- 全文大小：542 K
文摘
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The membrane from isolated sugar beet vacuoles was a valuable alternative to artificial membranes for investigating Syringopeptin 25A pore forming activity.
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The human channel TPC1 and the chloride/proton antiporter CLC-7 from endomembranes were functionally characterized using Arabidopsis tonoplast as a model membrane.
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We propose the plant vacuole as a novel system to study the molecular mechanisms of exogenous transporters and channels.