Topical interferon-gamma neutralization prevents conjunctival goblet cell loss in experimental murine dry eye

详细信息    查看全文

• 作者：Xiaobo Zhang ; Cintia S. De Paiva ; Zhitao Su ; Eugene A. Volpe ; De-Quan Li ; Stephen C. Pflugfelder
• 关键词：dry eye ; IFN-纬 ; apoptosis ; IL-13 ; FOXA2
• 刊名：Experimental Eye Research
• 出版年：January, 2014
• 年：2014
• 卷：118
• 期：Complete
• 页码：117-124
• 全文大小：2406 K

文摘

Evidence suggests that the cytokine interferon (IFN)-纬 released by natural killer and CD4⁺ T cells contributes to the conjunctival goblet cell (GC) loss in dry eye. The purpose of this study was to investigate if topical neutralization of IFN-纬 prevents or alleviates GC loss in an experimental desiccating stress (DS) model of dry eye. In this study, we found that topical IFN-纬 neutralization significantly decreased DS-induced conjunctival GC loss. This was accompanied by decreased epithelial apoptosis, and increased IL-13 and decreased FoxA2 expression in the forniceal conjunctiva. To establish that IFN-纬 produced by pathogenic CD4⁺ T cells contributes to DS-induced GC loss, adoptive transfer of CD4⁺ T cells isolated from DS exposed donors to na茂ve RAG-1^{鈭?鈭?/sup> recipient mice was performed. Similar to the donor mice, topical IFN-纬 neutralization decreased conjunctival GC loss, suppressed apoptosis and increased IL-13 expression in adoptive transfer recipients. In summary, this study demonstrated that topical neutralization of IFN-纬 prevents GC loss via modulating apoptosis and maintaining IL-13 signaling.}