HTLV-1 infects human mesenchymal stromal cell in vitro and modifies their phenotypic characteristics
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- 作者：Ev ; ra Strazza Rodrigues ; Mayra Dorigan de Macedo ; Mariana Tomazini Pinto ; Maristela Delgado Orellana ; Maur铆cio Cristiano Rocha Junior ; Danielle Aparecida Rosa de Magalh茫es ; Yuetsu Tanaka ; Osvaldo Massaiti Takayanagui ; Dimas Tadeu Covas ; Simone Kashima
- 关键词：MSC ; mesenchymal stromal cells ; BM ; bone marrow ; PBMC ; peripheral blood mononuclear cells ; HAM/TSP ; HTLV-associated myelopathy/tropical spastic paraparesis ; HAC ; HTLV asymptomatic carriers ; ATLL ; adult T-cell leukemia/lymphoma ; CMV ; cytomegalovirus ; HHV ; human herpesvirus ; HHV8 ; PPAR纬 ; peroxisome proliferator-activated receptors gamma ; RUNX-2 ; runt-related transcription factor-2 ; CD73 ; NT5E ; 5-nucleotidase ; ecto ; HLA-class I ; human major histocompatibility complex ; class I ; CD45 ; leukocyte comm
- 刊名：Virology
- 出版年：20 January, 2014
- 年：2014
- 卷：449
- 期：Complete
- 页码：190-199
- 全文大小：5702 K
文摘
The typical characteristics of mesenchymal stem cells (MSCs) can be affected by inflammatory microenvironment; however, the exact contribution of HTLV-1 to MSC dysfunction remains to be elucidated. In this study, we demonstrated that MSC cell surface molecules VCAM-1 and ICAM-1 are upregulated by contact with HTLV-1, and HLA-DR was most highly expressed in MSCs co-cultured with MT2 cells. The expression levels of VCAM-1 and HLA-DR were increased in MSCs cultured in the presence of PBMCs isolated from HTLV-1-infected symptomatic individuals compared with those cultured with cells from asymptomatic infected individuals or healthy subjects. HTLV-1 does not impair the MSC differentiation process into osteocytes and adipocytes. In addition, MSCs were efficiently infected with HTLV-1 in vitro through direct contact with HTLV-1-infected cells; however, cell-free virus particles were not capable of causing infection. In summary, HTLV-1 can alter MSC function, and this mechanism may contribute to the pathogenesis of this viral infection.