Soluble cysteine-rich tick saliva proteins Salp15 and Iric-1 from E. coli
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- 作者：Philipp Kolba ; b ; Jolanta Vorreitera ; Jü ; ri Habichtc ; Detlef Bentropd ; Reinhard Wallichc ; Michael Nassala ; nassal2@ukl.uni-freiburg.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：DsbA ; thiol ; disulfide interchange protein DsbA ; GB1 ; immunoglobulin binding domain B1 of streptococcal protein G ; eGFP ; enhanced green fluorescent protein ; GST ; glutathione-S-transferase ; HSQC ; heteronuclear single quantum coherence ; MBP ; maltose-binding protein ; OspC ; outer surface protein C ; Salp15 ; salivary protein of 15 ; kDa ; Trx ; thioredoxin
- 刊名：FEBS Open Bio
- 出版年：2015
- 出版时间：2015
- 年：2015
- 卷：5
- 期：Complete
- 页码：42-55
- 全文大小：3300 K
文摘
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Tick saliva proteins Salp15 and Iric-1 promote tick feeding and pathogen transmission.
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We established the first bacterial expression system for soluble Salp15 and Iric-1.
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Using this system we mapped monoclonal antibody epitopes on Salp15 and Iric-1.
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We defined the interaction sites with Borrelia outer surface protein C (OspC).
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We elucidated first secondary structure features in Iric-1 by NMR.